Batman has rarely managed to make the Joker flinch. Their tango continues, year after year, and the Joker keeps laughing because he knows that Batman will never do what it takes to cut the music and end the dance for good. -
But the Punisher isn't Batman. Joker learned this the hard way in the 1990s crossover issue Deadly Knights. -
Not many things can wipe the Joker's grin off his face, but Frank Castle had a .45 caliber trick up his sleeve. The Punisher isn't known for doling out second chances, especially for a homicidal maniac that has probably killed hundreds and will go on to kill thousands more. Joker recognised the conviction in the eyes of the man holding the gun, and for once realised the finality of his situation but then Batman came along at the perfect time to save The Joker and tells him to escape.